Journal: Structure (London, England : 1993)

Article Title: Structure of an RNA Aptamer that Can Inhibit HIV-1 by Blocking Rev-Cognate RNA (RRE) Binding and Rev-Rev Association

doi: 10.1016/j.str.2018.06.001

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: pET11a Rev 1–93 , Paul T. Wingfield (Genscript) , plasmid # 1979.

Techniques: Virus, Recombinant, Plasmid Preparation, Software, Control

Journal: Frontiers in Neurology

Article Title: Outer Plexiform Layer Structures Are Not Altered Following AAV-Mediated Gene Transfer in Healthy Rat Retina

doi: 10.3389/fneur.2017.00059

Figure Lengend Snippet: Primary antibodies .

Article Snippet: C-terminal-binding protein 2 (CtBP2) , rb , Polyclonal , 1:5,000 , RIBEYE in synaptic ribbons of photoreceptor terminals and bipolar cells, CtBP2 in cell nuclei , Synaptic-Systems, Goettingen, Germany , 193-003.

Techniques: Membrane

Journal: Frontiers in Neurology

Article Title: Outer Plexiform Layer Structures Are Not Altered Following AAV-Mediated Gene Transfer in Healthy Rat Retina

doi: 10.3389/fneur.2017.00059

Figure Lengend Snippet: Vertical cryosections of the retinas of adeno-associated virus (AAV)-transduced (left column) and untreated control animals (right column) with antibodies against different outer plexiform layer (OPL) structures . (A) AAV-transduced area in treated animal. The GFP fluorescence in the photoreceptors is clearly discernible. (B) Untreated control. (C,D) Higher magnification images of the areas highlighted in panels (A,B) with antibodies against C-terminal-binding protein 2 (CtBP2) (red) staining photoreceptor synaptic ribbons and calcium-binding protein (CaBP) (green) marking horizontal cells. (E,F) OPL of treated (left) and untreated (right) retinas with antibodies for DHP (red) marking voltage-gated calcium channels on invaginating bipolar cell dendritic tips and PKCα (green) marking rod bipolar cells. (G,H) OPL of treated (left) and control (right) retinas with antibodies for PSD95 (red) and CaBP (green). Structure and arrangement as well as overall number of synaptic contacts between first and second order neurons do not appear to be altered in treated retinas. n = 5 AAV-transduced eyes and n = 5 untreated controls. Abbreviations: compare Figure . Scale bar in panel (B) [for panels (A,B) ]: 20 µm; scale bar in panel (F) [for panels (C–H) ]: 10 µm.

Article Snippet: C-terminal-binding protein 2 (CtBP2) , rb , Polyclonal , 1:5,000 , RIBEYE in synaptic ribbons of photoreceptor terminals and bipolar cells, CtBP2 in cell nuclei , Synaptic-Systems, Goettingen, Germany , 193-003.

Techniques: Virus, Control, Fluorescence, Binding Assay, Staining

Journal: Frontiers in Neurology

Article Title: Outer Plexiform Layer Structures Are Not Altered Following AAV-Mediated Gene Transfer in Healthy Rat Retina

doi: 10.3389/fneur.2017.00059

Figure Lengend Snippet: Quantification of synapses in the outer plexiform layer (OPL) . Boxplots showing the mean number (±1 SD) of counted signals in the OPL for synaptic ribbons [C-terminal-binding protein 2 (CtBP2)] and bipolar cell dendritic tips (DHP) in adeno-associated virus (AAV)-transduced areas (transduced), areas sham-injected with fluorescein (sham), and untreated controls (control). n = 3 for AAV-transduced and fluorescein-injected eyes, and n = 6 for untreated control eyes.

Article Snippet: C-terminal-binding protein 2 (CtBP2) , rb , Polyclonal , 1:5,000 , RIBEYE in synaptic ribbons of photoreceptor terminals and bipolar cells, CtBP2 in cell nuclei , Synaptic-Systems, Goettingen, Germany , 193-003.

Techniques: Binding Assay, Virus, Injection, Control